Application
Western blot analysis of PP2A-B55δ expression in non-transfected 293T: (A), human PP2A-B55δ transfected 293T: (B) and KNRK (C) whole cell lysates.| Product Name | PP2A-B55 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 148-447 mapping at the C-terminus of PP2A-Bα of human origin |
| Modification Notes | In eukaryotes, the phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions, including division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the protein phosphatases. In general, the protein phosphatase (PP) holoenzyme is a trimeric complex composed of a regulatory subunit, a variable subunit and a catalytic subunit. Four major families of protein phosphatase catalytic subunits have been identified, designated PP1, PP2A, PP2B (calcineurin) and PP2C. An additional protein phosphatase catalytic subunit, PPX (also known as PP4) is a putative member of a novel PP family. The PP2A family comprises subfamily members PP2Aå and PP2A∫. The PP2A catalytic subunit associates with a variety of regulatory subunits. The B family of regulatory subunits (including B55, B56 and PR72/130 subfamilies) is believed to participate in substrate specificity and catalytic activity. PP2A-B55, also known as PP2A regulatory subunit subfamily B55 or PP2A-B1, is a B subfamily consisting of four B55 isoforms (å, ∫, © and ∂) encoded by four distinct genes. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of PP2A-Bα, PP2A-Bβ, PP2A-Bγ and PP2A-Bδ subunits of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 148-447 mapping at the C-terminus of PP2A-B&alpha of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 148-447 mapping at the C-terminus of PP2A-Bα of human origin |
| Entrez Gene | 5520 |
|---|
Application
Western blot analysis of PP2A-B55δ expression in non-transfected 293T: (A), human PP2A-B55δ transfected 293T: (B) and KNRK (C) whole cell lysates.| Application Notes | recommended for detection of PP2A-Bα, PP2A-Bβ, PP2A-Bγ and PP2A-Bδ subunits of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
