Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testes tissue showing cytoplasmic staining of cells in seminiferus ducts.Application
Western blot analysis of HSP 90 expression in non-transfected 293T: (A), human HSP 90 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of HSP 90α expression in non-transfected 293T: (A), mouse HSP 90α transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of human recombinant HSP 90α (A) and human recombinant HSP 90β (B) fusion proteins.Application
Western blot analysis of HSP 90 expression in HeLa (A) and NIH/3T3 (B) whole cell lysates.Application
Intracellular FCM analysis of fixed and permeabilized, heat shock-treated NIH/3T3 cells. Black line histogram represents the isotype control, normal rabbit IgG: .Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization.| Product Name | HSP 90α/β Antibody (H-114) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 610-723 mapping at the C-terminus of HSP 90β of human origin |
| Modification Notes | The heat shock response was first described for Drosophila salivary gland cells and morphologically consists of a change in their polytene chromosome puffing patterns that involves de novo synthesis of a few proteins. Similar heat shock proteins were later discovered in bacterial chicken and mammalian cells, and have been subsequently studied in other organisms. A series of proteins including HSP 90, HSP 70, HSP 20-30 and ubiquitin are induced by insults such as temperature shock, chemicals and other environmental stress. A major function of HSP 90 and other HSPs is to act as molecular chaperones. HSP 90 forms a complex with glucocorticoid receptor (GR), rendering the non ligand-bound receptor transcriptionally inactive. HSP 90 binds the GR as a heterocomplex composed of either HSP 56 or Cyclophilin D, forming an aporeceptor comiplex. HSP 90 also exists as a dimer with other proteins such as p60/sti1 and p23, forming an apo-receptor complex with estrogen and androgen receptors. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFFCELISA |
| recommended for detection of HSP 90α and HSP 90β of mouse, rat and human origin by WB, IP, IF, FCM and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 610-723 mapping at the C-terminus of HSP 90&beta of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 610-723 mapping at the C-terminus of HSP 90β of human origin |
| Entrez Gene | 3320 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testes tissue showing cytoplasmic staining of cells in seminiferus ducts.
Application
Western blot analysis of HSP 90 expression in non-transfected 293T: (A), human HSP 90 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of HSP 90α expression in non-transfected 293T: (A), mouse HSP 90α transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of human recombinant HSP 90α (A) and human recombinant HSP 90β (B) fusion proteins.
Application
Western blot analysis of HSP 90 expression in HeLa (A) and NIH/3T3 (B) whole cell lysates.
Application
Intracellular FCM analysis of fixed and permeabilized, heat shock-treated NIH/3T3 cells. Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization.| Application Notes | recommended for detection of HSP 90α and HSP 90β of mouse, rat and human origin by WB, IP, IF, FCM and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
