Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human bronchus tissue showing nuclear and cytoplasmic staining of respiratory epithelial cells.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human salivary gland tissue showing nuclear and cytoplasmic staining of glandular cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of Nurr1 and Nur77 expression in human Nur77 transfected 293: (A), non-transfected 293: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of Nurr1 expression in human Nurr1 transfected: (A) and non-transfected: (B) 293 whole cell lysates.Application
Western blot analysis of Nurr1/Nur77 expression in SK-N-MC whole cell lysate.| Product Name | Nurr1/Nur77 Antibody (E-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of Nurr1 of rat origin |
| Modification Notes | Nurr1 (Nur-related factor 1) and Nur77 (also designated NGFI-B) encode orphan nuclear receptors which may comprise an additional subfamily within the nuclear receptor superfamily. The rat and human homologs of mouse Nurr1 are designated RNR1 and NOT, respectively. Both NURR1 and Nur77 are growth factor inducible immediate early response genes. Induction of both Nurr1 and Nur77 is seen after membrane depolarization while only Nur77 induction is seen with NGF stimulation. JunD acts as a mediator for Nur77. An increase in Nur77 expression is seen in activated T cells during G0 to G1 transition and throughout the G1 phase. In addition to its function as an immediate early gene, Nur77 may play a role in TCR-mediated apoptosis. Cyclosporin A, a potent immuno-suppressant, has been shown to inhibit the ability of Nur77 to bind DNA. A dominant negative form of Nur77 can protect T cell hybridomas from activation-induced apoptosis. However, the absolute requirement of Nur77 for TCR-mediated apoptosis is still under debate. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of Nurr1 and Nur77 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of Nurr1 of rat origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of Nurr1 of rat origin |
| Entrez Gene | 4929 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human bronchus tissue showing nuclear and cytoplasmic staining of respiratory epithelial cells.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human salivary gland tissue showing nuclear and cytoplasmic staining of glandular cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of Nurr1 and Nur77 expression in human Nur77 transfected 293: (A), non-transfected 293: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of Nurr1 expression in human Nurr1 transfected: (A) and non-transfected: (B) 293 whole cell lysates.
Application
Western blot analysis of Nurr1/Nur77 expression in SK-N-MC whole cell lysate.| Application Notes | recommended for detection of Nurr1 and Nur77 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
