Application
Immunoperoxidase staining of formalin fixed paraffin embedded C57BL/6 mouse placenta showing cytoplasmic staining. Kindly provided by Jenna Tabor-Godwin, PhD Candidate and Dr. Ralph Feuer, San Diego State University.Application
Western blot analysis of CD73 expression in non-transfected: (A) and human CD73 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of CD73 expression in non-transfected 293: (A), human CD73 transfected 293: (B) and K-562 (C) whole cell lysates.| Product Name | CD73 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 275-574 mapping at the C-terminus of CD73 of human origin |
| Modification Notes | CD73 (also designated ecto-5'-nucleotidase, E5NT, NT, NT5, NTE, eN and eNT) is a glycosyl-phosphatidylinositol (GPI)-anchored adhesion protein that catalyzes the dephosphorylation of extracellular purine and pyrimidine nucleotides to their corresponding bioactive nucleosides. CD73 is a dimer of two identical subunits that depends on GPI to link with the external face of the plasma membrane. Similar to other GPI-anchored proteins, CD73 mediates co-stimulatory signals in T cell activation. CD73 has few structural variants, yet elicits diverse biological function through differential regulation in endothelial cells (EC), subpopulations of B and T cells, germinal center follicular dendritic cells and on thymic medullary reticular fibroblasts. For example, IgG mediated neutralization of CD73 interferes with lymphocyte adhesion to EC, and blocks aggregation of germinal center B cells and follicular dendritic cells. Furthermore, IgG-mediated targeting of lymphocyte CD73, but not of endothelial cell CD73, causes shedding of CD73 and tyrosine phosphorylation of proteins. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of 5'-nucleotidase of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 275-574 mapping at the C-terminus of CD73 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 275-574 mapping at the C-terminus of CD73 of human origin |
| Entrez Gene | 4907 |
|---|
Application
Immunoperoxidase staining of formalin fixed paraffin embedded C57BL/6 mouse placenta showing cytoplasmic staining. Kindly provided by Jenna Tabor-Godwin, PhD Candidate and Dr. Ralph Feuer, San Diego State University.
Application
Western blot analysis of CD73 expression in non-transfected: (A) and human CD73 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of CD73 expression in non-transfected 293: (A), human CD73 transfected 293: (B) and K-562 (C) whole cell lysates.| Application Notes | recommended for detection of 5'-nucleotidase of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
