Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.Application
Western blot analysis of CD83 expression in K-562 (A), CCRF-CEM (B), Jurkat (C) and MOLT-4 (D) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed Ramos cells showing membrane localization.| Product Name | CD83 Antibody (H-198) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 7-205 of CD83 of human origin |
| Conjugation | Agarose |
| Modification | Acetylated |
| Modification Notes | CD83 is a heavily glycosylated membrane protein of the immunoglobulin (Ig) superfamily that is expressed in mature dendritic cells, Langerhans cells and interdigitating reticulum cells within lymphoid tissues. Structurally, CD83 resembles other Ig superfamily members, which have an extracellular V-type Ig-like domain, a single transmembrane domain and a 40 residue cytoplasmic tail. CD83 expression is used as a marker for mature, antigen presenting dendritic cells that are capable of generating tumor-specific T cell immunity, a phenotype with implications as an anti-cancer vaccine. CD83-IgG1(fc) chimera studies indicate that CD83 is a sialic acid-binding, Ig-like Lectin (Siglec) adhesion molecule that is involved in cell adhesion/signaling by hosting dendritic cell interactions with monocytes and CD8+ T cells. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIPWB |
| WB:1:100~1:400 IP:1:20~1:100 IF:1:20~1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 7-205 of CD83 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 7-205 of CD83 of human origin |
| Entrez Gene | 9308 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.
Application
Western blot analysis of CD83 expression in K-562 (A), CCRF-CEM (B), Jurkat (C) and MOLT-4 (D) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed Ramos cells showing membrane localization.| Application Notes | WB:1:100~1:400 IP:1:20~1:100 IF:1:20~1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
