Application
Western blot analysis of CD71 expression in untreated (A), PMA-treated (B) and IL-2-treated (C) Jurkat whole cell lysates.| Product Name | CD71 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 461-760 mapping within the extracellular domain of CD71 of human origin |
| Modification Notes | CD71, also known as the transferrin receptor (TFR), is a type II membrane glycoprotein that exists as a disulfide-linked homodimer of two identical subunits. CD71 binds to two molecules of transferrin and a serum iron-transport protein, and directs the cellular uptake of iron via receptor-mediated endocytosis. CD71 is expressed, typically at high levels, on all proliferating cells, reticulocytes and erythroid precursors. It is not expressed on resting leukocytes, but is upregulated upon activation of lymphocytes, monocytes and macrophages. CD71 is also found on most dividing cells and on brain endothelium. A second transferrin receptor, TFR2, also mediates the uptake of transferrin-bound iron. TFR2 is a two-subunit homodimer and is highly expressed in liver as well as in hepatocytes and erythroid precursors. Mutations in the TFR2 gene result in hereditary hemochromatosis type III (HFE3), an iron overloading disorder predominant in Caucasians. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CD71 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 461-760 mapping within the extracellular domain of CD71 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 461-760 mapping within the extracellular domain of CD71 of human origin |
| Entrez Gene | 7037 |
|---|
Application
Western blot analysis of CD71 expression in untreated (A), PMA-treated (B) and IL-2-treated (C) Jurkat whole cell lysates.| Application Notes | recommended for detection of CD71 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
