Application
Western blot analysis of GFP fusion protein expression in non-transfected COS cells (A) and COS cells transfected with pCruz GFP-Lac Z: (B).Application
Fluorescence staining of methanol-fixed COS cells transfected with pCruz GFP-Lac Z: , showing GFP-Lac Z localization in the cytoplasm.| Product Name | GFP Antibody (FL) |
|---|---|
| Antibody Type | Tags Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-238 representing full length GFP (green fluorescent protein) of Aequorea victoria origin |
| Modification Notes | The green fluorescent protein (GFP) was originally identified as a protein involved in the bioluminescence of the jellyfish Aequorea victoria. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors, making GFP a useful tool for monitoring gene expression and protein localization in vivo. Several GFP mutants have been developed, including EGFP, which fluoresce more intensely than the wildtype GFP and have shifted excitation maxima, making them useful for FACS and fluorescence microscopy as well as double-labeling applications. GFP is widely used in expression vectors as a fusion protein tag, allowing expression and monitoring of heterologous proteins fused to GFP. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of GFP and GFP mutant fusion proteins by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-238 representing full length GFP (green fluorescent protein) of Aequorea victoria origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-238 representing full length GFP (green fluorescent protein) of Aequorea victoria origin |
Application
Western blot analysis of GFP fusion protein expression in non-transfected COS cells (A) and COS cells transfected with pCruz GFP-Lac Z: (B).
Application
Fluorescence staining of methanol-fixed COS cells transfected with pCruz GFP-Lac Z: , showing GFP-Lac Z localization in the cytoplasm.| Application Notes | recommended for detection of GFP and GFP mutant fusion proteins by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
