Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human bronchus tissue showing cytoplasmic staining of respiratory epithelial cells.Application
Western blot analysis of p120 expression in non-transfected 293T: (A), mouse p120 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Antibodies tested include p120 (15D2): (A) and p120 (H-90): (B,C).Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization.| Product Name | p120 Antibody (H-90) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 41-130 mapping near the N-terminus of p120 of human origin |
| Modification Notes | The catenins, a, b and g, are proteins which bind to the highly conserved, intra-cellular cytoplasmic tail of E-cadherin. Together, the catenin/cadherin complexes play an important role mediating cellular adhesion. a-catenin was initially described as an E-cadherin-associated protein and has been shown to associate with other members of the cadherin family, N-cadherin and P-cadherin. B-catenin associates with the cytoplasmic portion of E-cadherin which is necessary for the function of E-cadherin as an adhesion molecule. b-catenin has also been found in complexes with the tumor suppressor protein APC. g-catenin, also known as plakoglobin, is a protein that binds with a-catenin and N-cadherin. A related protein, p120, exhibits sequence homology with the catenins at four discreet domains. p120 not only serves as a substrate for Src, but is also found in E-cadherin complexes containing catenins. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of p120 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 41-130 mapping near the N-terminus of p120 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 41-130 mapping near the N-terminus of p120 of human origin |
| Entrez Gene | 1500 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human bronchus tissue showing cytoplasmic staining of respiratory epithelial cells.
Application
Western blot analysis of p120 expression in non-transfected 293T: (A), mouse p120 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Antibodies tested include p120 (15D2): (A) and p120 (H-90): (B,C).
Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization.| Application Notes | recommended for detection of p120 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
