Application
Western blot analysis of CD23 expression in non-transfected: (A) and human CD23 transfected: (B) 293T whole cell lysates and human platelet whole cell lysate (C).Application
Western blot analysis of CD23 expression in NIH/3T3 whole cell lysate immunoprecipitated with CD23 (FO14): and detected with CD23 (M-282): .Application
Western blot analysis of CD23 expression in BC3H1 (A), CTLL-2 (B), F9 (C), KNRK (D), NIH/3T3 (E) and RAW 264.7 (F) whole cell lysates.| Product Name | CD23 Antibody (M-282) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 50-331 mapping within the extracellular domain of CD23 of mouse origin |
| Modification Notes | The human leukocyte differentiation antigen CD23 (FCE2) is a type II integral membrane glycoprotein that is expressed on mature B cells, monocytes, eosinophils, platelets and dendritic cells. In mouse, CD23 is found only on mature B cells. CD23 is a low affinity IgE receptor that mediates IgE-dependent cytotoxicity and phagocytosis by macrophages and eosinophils. CD23 associates as an oligomer where cooperative binding of at least two lectin domains is required for high affinity IgE binding to CD23. It may play a role in antigen presentation by B cells by interacting with CD40. CD23 has been shown to be associated with the Fyn tyrosine kinase. The truncated molecule can be secreted, then function as a potent mitogenic growth factor. ADAM8, ADAM15, and MDC-L catalyze ectodomain shedding of CD23. Intestinal cells coexpress CD23a and CD23b, and the two splice forms show different localizations in polarized cells. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CD23 of mouse and rat origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 50-331 mapping within the extracellular domain of CD23 of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 50-331 mapping within the extracellular domain of CD23 of mouse origin |
| Entrez Gene | 14128 |
|---|
Application
Western blot analysis of CD23 expression in non-transfected: (A) and human CD23 transfected: (B) 293T whole cell lysates and human platelet whole cell lysate (C).
Application
Western blot analysis of CD23 expression in NIH/3T3 whole cell lysate immunoprecipitated with CD23 (FO14): and detected with CD23 (M-282): .
Application
Western blot analysis of CD23 expression in BC3H1 (A), CTLL-2 (B), F9 (C), KNRK (D), NIH/3T3 (E) and RAW 264.7 (F) whole cell lysates.| Application Notes | recommended for detection of CD23 of mouse and rat origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
