Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing Golgi and cytoplasmic localization.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human seminal vesicle tissue showing cytoplasmic staining of glandular cells in low (A) and high (B) resolution. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of C4α expression in non-transfected: (A) and human C4α transfected: (B) 293T whole cell lysates.Application
Western blot analysis of C4a expression in non-transfected: (A) and human C4a transfected: (B) 293T whole cell lysates.Application
Western blot analysis of C4a expression in mouse liver tissue extract.Application
Western blot analysis of C4α expression in Hep G2 whole cell lysate.| Product Name | C4α Antibody (H-155) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1146-1300 of C4 of human origin |
| Modification Notes | The complement component proteins C3, C4 and C5 are potent anaphylatoxins that are released during complement activation, a system of ligand-surface protein interactions specific to cells of hematopoietic lineage. These proteins belong to the å2-Macroglobulin family, but retain distinctive features including an anaphylatoxin domain and a netrin (NTR) domain. The human C4 gene is polymorphic at two loci, C4A and C4B, mapping to chromosome 6p21.3. C4A expresses the Rodgers (Rg) blood group Ag, while C4B expresses the Chido (Ch) blood group Ag. C4 is expressed as a precursor that is cleaved into å, ∫ and © chains, all of which are non-identical cleavage products. The å chain of C4 may be cleaved to produce an acidic isotype, C4a, which reacts with amino groups, and a basic isotype, C4b, which reacts with hydroxyl groups. Deficiency in the C4 gene is associated with autoimmune or immune complex disorders, such as systemic lupus erythematosus. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of C4 precursor, C4 αchain and C4b of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1146-1300 of C4 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1146-1300 of C4 of human origin |
| Entrez Gene | 720 |
|---|
Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing Golgi and cytoplasmic localization.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human seminal vesicle tissue showing cytoplasmic staining of glandular cells in low (A) and high (B) resolution. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of C4α expression in non-transfected: (A) and human C4α transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of C4a expression in non-transfected: (A) and human C4a transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of C4a expression in mouse liver tissue extract.
Application
Western blot analysis of C4α expression in Hep G2 whole cell lysate.| Application Notes | recommended for detection of C4 precursor, C4 αchain and C4b of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
