Application
Immunofluorescence staining of normal mouse kidney frozen section showing cytoplasmic staining.| Product Name | p15/p16 Antibody (H-43) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 96-138 mapping at the C-terminus of p15 of human origin |
| Modification Notes | The normal progression of cells through the cell cycle is under the control of the cyclin-dependent protein kinases Cdk4 and Cdk6, which are subject to inhibition by the mitotic inhibitory protein p16. The complexes formed by Cdk4 and the D-type cyclins have been strongly implicated in the control of cell proliferation during the G1 phase. It has been shown that p16 binds to Cdk4 and inhibits the catalytic activity of the Cdk4/cyclin D complex. Moreover, the gene encoding p16 exhibits a high frequency of homozygous deletions and point mutations in established human tumor cell lines. Expression of p15, a member of the p16 family, is upregulated approximately 30-fold in TGF∫-treated human keratinocytes, suggesting that p15 may act as an effector of TGF∫-mediated cell cycle arrest. The gene encoding p15 has been mapped to chromosome 9p21, adjacent to the p16 gene, at a site of frequent chromosomal abnormality in human tumors. It has been suggested that p15 may function as an effector of TGF∫-mediated cell cycle arrest through inhibition of Cdk4 and Cdk6 kinases. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of p15 and p16 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 96-138 mapping at the C-terminus of p15 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 96-138 mapping at the C-terminus of p15 of human origin |
| Entrez Gene | 1029 |
|---|
Application
Immunofluorescence staining of normal mouse kidney frozen section showing cytoplasmic staining.| Application Notes | recommended for detection of p15 and p16 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
