Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.Application
Antibodies tested include Ac-Histone H3 (Lys 9/14): (A,B) and Histone H3 (C-16): (C,D).Application
Western blot analysis of Ac-Histone H3 acetylation in untreated (A,C) and Trichostatin A treated (B,D) NIH/3T3 whole cell lysates. Antibodies tested include: Ac-Histone H3 (Lys 24): (A,B) and Histone H3 (C-16): (C,D).| Product Name | Ac-Histone H3 Antibody (Lys 24) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | raised against a short amino acid sequence containing acetylated lysine 24 of Histone H3 of human origin |
| Modification Notes | In eukaryotes, DNA is wrapped around histone octamers to form the basic unit of chromatin structure. The octamer is composed of histones H2A, H2B, H3 and H4, and it associates with approximately 200 base pairs of DNA to form the nucleosome. The association of DNA with histones results in dense packing of chromatin, which restricts proteins involved in gene transcription from binding to DNA. p300 preferentially acetylates Histone H3 at lysines 14 and 18 and Histone H4 at lysines 5 and 8. PCAF in its native form, primarily acetylates Histone H3 at lysine 14 to a monoacetylated form, and less efficiently acetylates Histone H4 at lysine 8. Histone H4 may also be acetylated at lysines 12 and 16, and the involvement of acetylated H4 with Histones H2A, H2B and H3 suggests that acetylated histones may be involved in dynamic chromatin remodeling. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Lysine 24 Acetylated Histone H3 of broad origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against a short amino acid sequence containing acetylated lysine 24 of Histone H3 of human origin |
|---|---|
| Tissue Specificity | raised against a short amino acid sequence containing acetylated lysine 24 of Histone H3 of human origin |
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
Application
Antibodies tested include Ac-Histone H3 (Lys 9/14): (A,B) and Histone H3 (C-16): (C,D).
Application
Western blot analysis of Ac-Histone H3 acetylation in untreated (A,C) and Trichostatin A treated (B,D) NIH/3T3 whole cell lysates. Antibodies tested include: Ac-Histone H3 (Lys 24): (A,B) and Histone H3 (C-16): (C,D).| Application Notes | recommended for detection of Lysine 24 Acetylated Histone H3 of broad origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
