Application
Western blot analysis of phosphorylated HDAC4/5/9 expression in untreated (A) and Insulin-treated (B) 3T3 cell extracts.| Product Name | p-HDAC4/5/9 Antibody (Ser 246/259/220) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Ser 246 phosphorylated HDAC4 of human origin and correspondingly phosphorylated Ser 245 of mouse origin; Ser 259 phosphorylated HDAC5 of human origin and correspondingly phosphorylated Ser 250 of mouse origin and Ser 200 phosph |
| Modification | Phosphoralated |
| Modification Notes | In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. The histone deacetylases (HDAC) include HDAC1–9. Subject to post-translational modifications, HDAC4, 5 and 9 contain phosphoserine residues at Ser-246, Ser-259 and Ser-220, respectively. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIP |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Ser 246 phosphorylated HDAC4 of human origin and correspondingly phosphorylated Ser 245 of mouse origin Ser 259 phosphorylated HDAC5 of human origin and correspondingly phosphorylated Ser 250 of mouse origin and Ser 200 phosph |
|---|---|
| Tissue Specificity | recommended for detection of Ser 246 phosphorylated HDAC4 of human origin and correspondingly phosphorylated Ser 245 of mouse origin; Ser 259 phosphorylated HDAC5 of human origin and correspondingly phosphorylated Ser 250 of mouse origin and Ser 200 phosphorylated HDAC9 of human and mouse origin by WB and IP |
| Entrez Gene | 9734 |
|---|
Application
Western blot analysis of phosphorylated HDAC4/5/9 expression in untreated (A) and Insulin-treated (B) 3T3 cell extracts.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
