Application
Western blot analysis of AChRb1 expression in human AChRb1 non-transfected: (A) and transfected: (B) 293 whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded mouse brain tissue showing membrane localization.Application
Western blot analysis of AChRβ1 expression in IMR-32 whole cell lysate.| Product Name | AChRβ1 Antibody (H-101) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 356-456 of AChRβ1 of human origin |
| Modification Notes | Members of the ligand-gated ion channel receptor family are characterized by their fast transmitting response to neurotransmitters. Two important members of this family are the nicotinic acetylcholine and glutamate receptors, both of which are composed of five homologous subunits forming a transmembrane aqueous pore. These transmembrane receptors change conformation in response to their cognate neurotransmitter. Nicotinic acetylcholine receptors (AChRs) are found at the postsynaptic membrane of the neuromuscular junction and bind acetylcholine molecules, allowing ions to move through the pore. Glutamate receptors are found in the postsynaptic membrane of cells in the central nervous system. The activity that is generated at the synapse by the binding of acetylcholine is terminated by acetylcholinesterase, an enzyme that rapidly hydrolyzes acetylcholine. AChR∫1, also known as CHRNB, CMS1D, CMS2A, SCCMS or CHRNB1, is a 501 amino acid protein that belongs to the ligand-gated ionic channel family. Defects in the gene encoding AChR∫1 may be the cause of congenital myasthenic syndrome slow-channel type (SCCMS), which is characterized by muscle weakness affecting the axial and limb muscles, the ocular muscles and the facial and bulbar musculature. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of AChRβ1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 356-456 of AChR&beta 1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 356-456 of AChRβ1 of human origin |
| Entrez Gene | 1140 |
|---|
Application
Western blot analysis of AChRb1 expression in human AChRb1 non-transfected: (A) and transfected: (B) 293 whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded mouse brain tissue showing membrane localization.
Application
Western blot analysis of AChRβ1 expression in IMR-32 whole cell lysate.| Application Notes | recommended for detection of AChRβ1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
