Application
Western blot analysis of 14-3-3 ζ expression in non-transfected 293T: (A), human 14-3-3 ζ transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of 14-3-3 ζ expression in non-transfected 293T: (A), human 14-3-3 ζ transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of 14-3-3 ζ expression in non-transfected: (A) and human 14-3-3 ζ transfected: (B) 293T whole cell lysates.Application
Western blot analysis of 14-3-3 expression in non-transfected 293T: (A), human 14-3-3 transfected 293T: (B) and KNRK (C) whole cell lysates.Application
Western blot analysis of 14-3-3 ζ expression in non-transfected: (A) and human 14-3-3 ζ transfected: (B) 293T whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in glomeruli and tubuli. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of 14-3-3 ζ expression in NIH/3T3 (A), c4 (B), EOC 20 (C), CTLL-2 (D), 3T3-L1 (E) and WEHI-231 (F) whole cell lysates.Application
Western blot analysis of 14-3-3 ζ expression in non-transfected control (A) and 14-3-3 ζ siRNA transfected (B) HeLa cells. Blot probed with 14-3-3 ζ (C-16): . Actin (I-19): used as specificity and loading control.Application
Western blot analysis of 14-3-3 expression in KNRK whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast tumor (A) and immunofluorescence staining of methanol-fixed KNRK cells (B) showing cytoplasmic staining.| Product Name | 14-3-3 ζ Antibody (C-16) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping within a relatively divergent domain of 14-3-3 ζ of human origin |
| Modification Notes | 14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms comprise this family of signaling intermediates, denoted 14-3-3 ∫, ©, é, Ω, ñ, † and s. 14-3-3 proteins form dimers that present two binding sites for ligand proteins, thereby bringing together two proteins that may not otherwise associate. These ligands largely share a 14-3-3 consensus binding motif and exhibit serine/threonine phosphorylation. 14-3-3 proteins function in broad regulation of these ligand proteins, by cytoplasmic sequestration, occupation of interaction domains and import/export sequences, prevention of degradation, activation/repression of enzymatic activity and facilitation of protein modification, and thus loss of expression contributes to a vast array of pathogenic cellular activities. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of 14-3-3 ζand, to a lesser extent, 14-3-3 β and σ of mouse, rat, human and ovine origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within a relatively divergent domain of 14-3-3 &zeta of human origin |
|---|---|
| Tissue Specificity | epitope mapping within a relatively divergent domain of 14-3-3 ζ of human origin |
| Entrez Gene | 7534 |
|---|
Application
Western blot analysis of 14-3-3 ζ expression in non-transfected 293T: (A), human 14-3-3 ζ transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of 14-3-3 ζ expression in non-transfected 293T: (A), human 14-3-3 ζ transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of 14-3-3 ζ expression in non-transfected: (A) and human 14-3-3 ζ transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of 14-3-3 expression in non-transfected 293T: (A), human 14-3-3 transfected 293T: (B) and KNRK (C) whole cell lysates.
Application
Western blot analysis of 14-3-3 ζ expression in non-transfected: (A) and human 14-3-3 ζ transfected: (B) 293T whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in glomeruli and tubuli. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of 14-3-3 ζ expression in NIH/3T3 (A), c4 (B), EOC 20 (C), CTLL-2 (D), 3T3-L1 (E) and WEHI-231 (F) whole cell lysates.
Application
Western blot analysis of 14-3-3 ζ expression in non-transfected control (A) and 14-3-3 ζ siRNA transfected (B) HeLa cells. Blot probed with 14-3-3 ζ (C-16): . Actin (I-19): used as specificity and loading control.
Application
Western blot analysis of 14-3-3 expression in KNRK whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast tumor (A) and immunofluorescence staining of methanol-fixed KNRK cells (B) showing cytoplasmic staining.| Application Notes | recommended for detection of 14-3-3 ζand, to a lesser extent, 14-3-3 β and σ of mouse, rat, human and ovine origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
