Application
Antibodies tested include p-AMPKβ1 (Ser 108): (A,C) and AMPKβ1 (Z14): (B,D).| Product Name | p-AMPKβ1 Antibody (Ser 108) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to phosphorylated Ser 108 of AMPKβ1 of human origin |
| Modification | Phosphoralated |
| Modification Notes | Five-prime-AMP-activated protein kinase, known as AMPK, is a heterotrimeric complex that comprises of a catalytic å subunit, and regulatory ∫ and ©. AMPK protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. AMPK is activated by high AMP and low ATP via a mechanism involving allosteric regulation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase (AMPKK), and inhibition of dephosphorylation. Activated AMPK can phosphorylate and regulate in vivo hydroxymethylglutaryl-CoA reductase and acetyl-CoA carboxylase, which are key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively. The native ∫1 subunit has been found to be phosphorylated in vivo at three sites, Ser24/25, Ser108 and Ser182. Serine 108 is the major autophosphorylation site on the AMPK∫ subunit and mutation at this site leads to the inhibition of AMPK. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Ser 108 phosphorylated AMPKβ1 of mouse, rat and human origin by WB, IP, IF and ELISA may cross-react with correspondingly phosphorylated AMPKβ2 also reactive with additional species, including canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Ser 108 of AMPK&beta 1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Ser 108 of AMPKβ1 of human origin |
| Entrez Gene | 5564 |
|---|
Application
Antibodies tested include p-AMPKβ1 (Ser 108): (A,C) and AMPKβ1 (Z14): (B,D).| Application Notes | recommended for detection of Ser 108 phosphorylated AMPKβ1 of mouse, rat and human origin by WB, IP, IF and ELISA may cross-react with correspondingly phosphorylated AMPKβ2 also reactive with additional species, including canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
