Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and nuclear localization.Application
Western blot analysis of nm23-H1, nm23-H2 and nm23-H3 expression in BJAB (A) and A-431 (B) whole cell lysates.| Product Name | nm23-H1/2/3 Antibody (FL-152) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-152 representing full length nm23-H1 of human origin |
| Modification Notes | The nm23 protein is implicated in a variety of biological processes, including suppressing metastasis, phytochrome responses in plants and regulating differentiation. Nm23-H1, nm23-H2 and nm23-H2, members of the nm23 family, are putative metastasis suppressor genes that encode nucleoside diphosphate kinase (NDPK) A and B. NDPKs form oligomers, which are distributed in both the soluble and particulate fractions of cells (1,2). Upon cAMP-induced differentiation of rat cells, nm23 proteins show a differential interaction with intermediate filaments (3). Both isoforms also associate with GFAP in differentiated cells (3). nm23 proteins and their mutants are localized predominantly in the mitochondria. Nm23 mutants are unable to inhibit differentiation and promote apoptosis as a results of defective protein-protein interactions in the mitochondria (4). Nm23-H1, nm23-H2 and nm23-H3 are indicators of a poor prognosis in human hematopoietic malignancies, and as a high expression of nm23-H1 and -H2 is positively correlated with histological differentiation (5,6). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIFELISA |
| recommended for detection of nm23-H1, nm23-H2, nm23-H3 and to a lesser extent, nm23-H4 of mouse, rat and human origin by WB, IF and ELISA not recommended for IP: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-152 representing full length nm23-H1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-152 representing full length nm23-H1 of human origin |
| Entrez Gene | 4830 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and nuclear localization.
Application
Western blot analysis of nm23-H1, nm23-H2 and nm23-H3 expression in BJAB (A) and A-431 (B) whole cell lysates.| Application Notes | recommended for detection of nm23-H1, nm23-H2, nm23-H3 and to a lesser extent, nm23-H4 of mouse, rat and human origin by WB, IF and ELISA not recommended for IP: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
