Application
Western blot analysis of PKC ζ expression in non-transfected: (A) and human PKC ζ transfected: (B) 293 whole cell lysates.Application
Western blot analysis of PKC ζ expression in 293T whole cell lysate.Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.Application
PKC antibodies used include PKC α (C-20), PKC βI (C-16), PKC βII (C-18), PKC γ (C-19), PKC ε (C-15), PKC ζ (C-20) and PKC η (C-15).Application
Western blot analysis of PKC ζ expression in NIH/3T3 (A), 3611-RF (B) and A-431 (C) whole cell lysates.| Product Name | PKC ζ Antibody (C-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of PKC ζ of rat origin |
| Modification Notes | Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into many different isoforms (å, ∫I, ∫II, ©, ∂, é, Ω, h, †, λ/î, µ and n). Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of PKC ∂ and é are independent of Ca++. On the other hand, most of the other PKC members possess phorbol ester-binding activities and kinase activities. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of PKC ζ, PKC λ and PKC ι of mouse, rat, human, zebrafish, Drosophila and Xenopus origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of PKC &zeta of rat origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of PKC ζ of rat origin |
| Entrez Gene | 5590 |
|---|
Application
Western blot analysis of PKC ζ expression in non-transfected: (A) and human PKC ζ transfected: (B) 293 whole cell lysates.
Application
Western blot analysis of PKC ζ expression in 293T whole cell lysate.
Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.
Application
PKC antibodies used include PKC α (C-20), PKC βI (C-16), PKC βII (C-18), PKC γ (C-19), PKC ε (C-15), PKC ζ (C-20) and PKC η (C-15).
Application
Western blot analysis of PKC ζ expression in NIH/3T3 (A), 3611-RF (B) and A-431 (C) whole cell lysates.| Application Notes | recommended for detection of PKC ζ, PKC λ and PKC ι of mouse, rat, human, zebrafish, Drosophila and Xenopus origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
