Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing cytoplasmic localization.Application
Western blot analysis of CMBL expression in HeLa whole cell lysate.Application
Western blot analysis of CMBL expression in Hep G2 whole cell lysate.| Product Name | CMBL Antibody (FL-245) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-245 representing full length CMBL of human origin |
| Modification Notes | CMBL (Carboxymethylenebutenolidase homolog) is a 245 amino acid cytosolic protein and cysteine hydrolase that preferentially cleaves cyclic esters. A member of the dienelactone hydrolase family, CMBL is the human homolog of Pseudomonas dienelactone hydrolase, a protein that participates in the bacterial halocatechol degradation pathway. CMBL is ubiquitously expressed with high expression in liver, colon, kidney and small intestine, where it acts as a bioactivating enzyme for a prodrug type angiotensin II type 1 receptor antagonist known as olmesartan medoxomil. CMBL is inhibited by p-chloromercuribenzoate (PCMB) and is encoded by a gene that maps to human chromosome 5p15.2. Chromosome 5 contains 181 million base pairs and comprises nearly 6% of the human genome. Deletion of the p arm of chromosome 5 leads to Cri du chat syndrome, while deletion of the q arm, or of chromosome 5 altogether, is common in therapy-related acute myelogenous leukemias and myelodysplastic syndrome. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CMBL of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-245 representing full length CMBL of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-245 representing full length CMBL of human origin |
Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing cytoplasmic localization.
Application
Western blot analysis of CMBL expression in HeLa whole cell lysate.
Application
Western blot analysis of CMBL expression in Hep G2 whole cell lysate.| Application Notes | recommended for detection of CMBL of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
