Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing cytoplasmic localization.Application
Western blot analysis of C4a expression in non-transfected: (A) and human C4a transfected: (B) 293T whole cell lysates.Application
Western blot analysis of C4a expression in non-transfected: (A) and human C4a transfected: (B) 293T whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human breast cancer tissue showing cytoplasmic staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of C4β expression in mouse liver tissue extract.| Product Name | C4β Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 376-675 of C4 of human origin |
| Modification Notes | The complement component proteins C3, C4 and C5 are potent anaphylatoxins that are released during complement activation, a system of ligand-surface protein interactions specific to cells of hematopoietic lineage. These proteins belong to the å2-Macroglobulin family, but retain distinctive features including an anaphylatoxin domain and a netrin (NTR) domain. The human C4 gene is polymorphic at two loci, C4A and C4B, mapping to chromosome 6p21.3. C4A expresses the Rodgers (Rg) blood group Ag, while C4B expresses the Chido (Ch) blood group Ag. C4 is expressed as a precursor that is cleaved into å, ∫ and © chains, all of which are non-identical cleavage products. The å chain of C4 may be cleaved to produce an acidic isotype, C4a, which reacts with amino groups, and a basic isotype, C4b, which reacts with hydroxyl groups. Deficiency in the C4 gene is associated with autoimmune or immune complex disorders, such as systemic lupus erythematosus. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of C4 precursor and C4 β chain of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 376-675 of C4 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 376-675 of C4 of human origin |
| Entrez Gene | 720 |
|---|
Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing cytoplasmic localization.
Application
Western blot analysis of C4a expression in non-transfected: (A) and human C4a transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of C4a expression in non-transfected: (A) and human C4a transfected: (B) 293T whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human breast cancer tissue showing cytoplasmic staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of C4β expression in mouse liver tissue extract.| Application Notes | recommended for detection of C4 precursor and C4 β chain of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
