Application
Western blot analysis of Gβ expression in Y79 whole cell lysate.Application
Western blot analysis of Gβ 1 expression in non-transfected 293T: (A), mouse Gβ 1 transfected 293T: (B) and Jurkat (C) whole cell lysates.Application
Western blot analysis of Gβ expression in mouse brain tissue extract.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human salivary gland tissue showing cytoplasmic and membrane staining of glandular cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of normal mouse intestine frozen section showing membrane staining.Application
Western blot analysis of Gβ 4 expression in non-transfected 293T: (A), human Gβ 4 transfected 293T: (B) and MCP-5 (C) whole cell lysates.| Product Name | Gβ Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 mapping at the N-terminus of Gβ 2 of human origin |
| Modification Notes | Heterotrimeric G proteins transduce extracellular signals received by transmembrane receptors to effector proteins (1). Each subunit of the G protein complex is encoded by a member of 1 of 3 corresponding gene families (å, ∫, ©) and in mammals there are 5 different members of the ∫-subunit family (1). The ∫ subunits of the G proteins are important regulators of G protein a subunits as well as of certain signal transduction receptors and effectors (2). The gene which encodes transducin ∫1 maps to human chromosome 1pter-p31.2 (3,4). The gene which encodes transducin ∫2 maps to human chromsome 7q21 (4,5). The gene which encodes transducin ∫3 maps to human chromosome 12p13 (6). In contrast to ∫1-4, which are at least 83% homologous, transducin ∫5 is only 50% homologous to the other ∫ subunits (7). Human transducin ∫5 is expressed at high levels in brain, pancreas, kidney, and heart (7). The gene which encodes transducin ∫5 maps to human chromsome 15 (2). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Gβ 1, Gβ 2, Gβ 3 and Gβ 4 and, to a lesser extent, Gβ 5 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of G&beta 2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of Gβ 2 of human origin |
| Entrez Gene | 59345 |
|---|
Application
Western blot analysis of Gβ expression in Y79 whole cell lysate.
Application
Western blot analysis of Gβ 1 expression in non-transfected 293T: (A), mouse Gβ 1 transfected 293T: (B) and Jurkat (C) whole cell lysates.
Application
Western blot analysis of Gβ expression in mouse brain tissue extract.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human salivary gland tissue showing cytoplasmic and membrane staining of glandular cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of normal mouse intestine frozen section showing membrane staining.
Application
Western blot analysis of Gβ 4 expression in non-transfected 293T: (A), human Gβ 4 transfected 293T: (B) and MCP-5 (C) whole cell lysates.| Application Notes | recommended for detection of Gβ 1, Gβ 2, Gβ 3 and Gβ 4 and, to a lesser extent, Gβ 5 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
