| Product Name | ANKRD28/44/52 Antibody (H-135) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 262-394 mapping within an internal region of ANKRD44 of human origin |
| Modification Notes | Ankyrins are membrane adaptor molecules that play important roles in coupling integral membrane proteins to the spectrin-based cytoskeleton network. Mutations of ankyrin genes lead to severe genetic diseases, such as fatal cardiac arrhythmias and hereditary spherocytosis. ANKRD28 (Ankyrin repeat domain-containing protein 28), also known as PITK, is a 1,086 amino acid protein that localizes to the nucleus and contains 27 ankyrin repeats. Expressed in a variety of tissues, two isoforms of ANKRD28 exist due to alternative splicing events. ANKRD44 (ankyrin repeat domain 44), also known as PP6-ARS-B (serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit B), is a 993 amino acid protein that contains twenty-eight ANK repeats and exists as five alternatively spliced isoforms. ANKRD44 is conserved in chimpanzee, dog, mouse, rat, chicken, zebrafish and Magnaporthe grisea. ANKRD52 (ankyrin repeat domain 52), also known as PP6-ARS-C (serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit C), is a 1,076 amino acid phosphoprotein that contains twenty-eight ANK repeats. ANKRD52 is conserved in chimpanzee, dog, cow, mouse, rat, chicken and zebrafish. ANKRD28/44/52 are putative regulatory subunits of protein phospatase 6 (PP6), a holoenzyme that may be a heterotrimeric complex formed by a catalytic subunit, a SKAP55 domain-containing subunit (PP6R) and an ankyrin repeat-domain containing regulatory subunit (ARS). ANKRD28/44/52 may also be involved in phosphoprotein substrate recognition. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of ANKRD28 of mouse, rat and human origin, ANKRD52 of human origin and ANKRD44 of mouse and human origin by WB, IP, IF and ELISA non cross-reactive with other ANKRD family members also reactive with additional species, including equine, canine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 262-394 mapping within an internal region of ANKRD44 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 262-394 mapping within an internal region of ANKRD44 of human origin |
| Entrez Gene | 91526 |
|---|
| Application Notes | recommended for detection of ANKRD28 of mouse, rat and human origin, ANKRD52 of human origin and ANKRD44 of mouse and human origin by WB, IP, IF and ELISA non cross-reactive with other ANKRD family members also reactive with additional species, including equine, canine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
