Application
Western blot analysis of Akt1 phosphorylation in non-transfected: (A) and human Akt1 transfected: (B) 293T whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic staining.Application
Western blot analysis of phosphorylated p-Akt1 expression in untreated (A) and heat shock-treated (B) HeLa whole cell lysates.| Product Name | p-Akt1/2/3 Antibody (Ser 473) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to phosphorylated Ser 473 of Akt1 of human origin |
| Modification | Phosphoralated |
| Modification Notes | The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 (also designated PKB∫ or RacPK-∫) and Akt 3 (also designated PKB© or thyoma viral proto-oncogene 3), which exhibit sequence homology with the protein kinase A and C families and are encoded by the c-Akt proto-oncogene. All members of the Akt family have a Pleckstrin homology domain. Akt1 and Akt2 are activated by PDGF stimulation. This activation is dependent on PDGFR-∫ tyrosine residues 740 and 751, which bind the subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1(IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Akt proteins become phosphorylated and activated in insulin/IGF-1-stimulated cells by an upstream kinase(s), and the activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor Wortmannin. Taken together, this data strongly suggests that the protein signals downstream of the PI kinases. Akt3 is phosphorylated on a serine residue in response to insulin. However, the activation of Akt3 by insulin is inhibited by prior activation of protein kinase C via a mechanism that does not require the presence of the PH domain. Akt3 is expressed in 3T3-L1 fibroblasts, adipocytes and skeletal muscle and may be involved in various biological processes, including adipocyte and muscle differentiation, glycogen synthesis, glucose uptake, apoptosis and cellular proliferation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC |
| recommended for detection of Akt1 phosphorylated Ser 473 and correspondingly Ser 474 phosporylated Akt2 and Ser 472 phosporylated Akt3 of mouse, rat and human origin by WB, IP, IF and IHC(P): |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Ser 473 of Akt1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Ser 473 of Akt1 of human origin |
| Entrez Gene | 207 |
|---|
Application
Western blot analysis of Akt1 phosphorylation in non-transfected: (A) and human Akt1 transfected: (B) 293T whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic staining.
Application
Western blot analysis of phosphorylated p-Akt1 expression in untreated (A) and heat shock-treated (B) HeLa whole cell lysates.| Application Notes | recommended for detection of Akt1 phosphorylated Ser 473 and correspondingly Ser 474 phosporylated Akt2 and Ser 472 phosporylated Akt3 of mouse, rat and human origin by WB, IP, IF and IHC(P): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
