Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human epididymis tissue showing cytoplasmic staining of glandular cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.| Product Name | PGD2 synthase Antibody (FL-190) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-190 representing full length PGD2 synthase of human origin |
| Modification Notes | Human PGD synthase is the key enzyme for production of the D and J series of prostanoids in the immune system and mast cells. This enzyme is the first member of the sigma class glutathione S-transferases (GST) from vertebrates and contains a prominent cleft as the active site, which is unique among members of the GST superfamily. The human PGD synthase gene, which maps to chromosome 4q22.3, is expressed in a species-specific manner. For instance, the human gene is widely distributed, whereas the mouse gene is only expressed in oviduct and skin. Human PGD synthase is expressed in the cytoplasm of human megakaryoblastic CMK cells prior to differentiation into platelets, which have no PGD synthase activity. Another member of the PGD synthase family, PGD2 synthase, catalyzes the conversion of PGH2 to PGD2 and is essential for the synthesis of PGD2 in the brain. Unlike PGD synthase, PGD2 synthase is not dependent on the presence of glutathione for its activity. The human PGD2 synthase gene maps to chromosome 9q34.3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of PGD2 synthase of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-190 representing full length PGD2 synthase of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-190 representing full length PGD2 synthase of human origin |
| Entrez Gene | 5730 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human epididymis tissue showing cytoplasmic staining of glandular cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.| Application Notes | recommended for detection of PGD2 synthase of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
