Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane and cytoplasmic localization.Application
Western blot analysis of IQGAP1 expression in non-transfected 293T: (A), mouse IQGAP1 transfected 293T: (B) and TT (C) whole cell lysates.Application
Western blot analysis of IQGAP1 expression in A549 (A) and Caki-1 (B) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed KNRK cells showing cytoplasmic and membrane staining.Application
Western blot analysis of IQGAP1 expression in KNRK whole cell lysate.| Product Name | IQGAP1 Antibody (H-109) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 314-422 mapping near the N-terminus of IQGAP1 of human origin |
| Modification Notes | IQGAP1, for IQ motif containing GTPase activating protein, is a Ras GAP-related, Actin-binding protein that interacts with the small GTPases Cdc42 and Rac1. The C-terminus of IQGAP1 is essential for interacting with Cdc42 and, in addition, IQGAP1 contains a WW domain and a predicted N-terminal coiled-coil region, which may be involved in IQGAP dimerization. Expression of IQGAP1 is highest in placenta, lung and kidney, where it co-localizes with Cdc42 to the cytoskeleton and assists with Cdc42 in mediating the regulation of cell proliferation, polarity and cell morphology. IQGAP1 regulates cadherin-mediated cell adhesion via binding to E-cadherin, b-catenin and a-catenin. This association induces the accumulation of these proteins at the site of cell-cell contact. IQGAP1 is negatively regulated by calmodulin, which binds to IQGAP1 in a calcium-dependent manner and disrupts IQGAP1 from associating with Cdc42. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of IQGAP1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 314-422 mapping near the N-terminus of IQGAP1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 314-422 mapping near the N-terminus of IQGAP1 of human origin |
| Entrez Gene | 8826 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane and cytoplasmic localization.
Application
Western blot analysis of IQGAP1 expression in non-transfected 293T: (A), mouse IQGAP1 transfected 293T: (B) and TT (C) whole cell lysates.
Application
Western blot analysis of IQGAP1 expression in A549 (A) and Caki-1 (B) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed KNRK cells showing cytoplasmic and membrane staining.
Application
Western blot analysis of IQGAP1 expression in KNRK whole cell lysate.| Application Notes | recommended for detection of IQGAP1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
