Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of exocrine pancreas and islet cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of normal mouse intestine frozen section showing membrane and cytoplasmic staining.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of N-Ras expression in HeLa (A) and KNRK (B) whole cell lysates.| Product Name | N-Ras Antibody (C-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of N-Ras of human origin |
| Modification Notes | The mammalian Ras (also designated v-Ha-Ras, Harvey rat sarcoma viral oncogene homolog, HRAS1, K-Ras, N-Ras, RASH1 or c-bas/has) gene family consists of the Harvey and Kirsten Ras genes (c-H-Ras1 and c-K-Ras2), an inactive pseudogene of each (c-H-Ras2 and c-K-Ras1) and the N-Ras gene. The three Ras oncogenes, H-Ras, K-Ras and N-Ras, encode proteins with GTP/GDP binding and GTPase activity. Ras proteins alternate between an inactive form bound to GDP and an active form bound to GTP, activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP). Ras nomenclature originates from the characterization of human DNA sequences homologous to cloned DNA fragments containing oncogenic sequences of a type C mammalian retrovirus, the Harvey strain of murine sarcoma virus (HaMSV), derived from the rat. Under normal conditions, Ras family members influence cell growth and differentiation events in a subcellular membrane compartmentalization-based signaling system. Oncogenic Ras can deregulate processes that control both cell proliferation and apoptosis. The Ras superfamily of GTP hydrolysis-coupled signal transduction relay proteins can be subclassified into Ras, Rho, Rab and ARF families. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of N-Ras p21 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of N-Ras of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of N-Ras of human origin |
| Entrez Gene | 4893 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of exocrine pancreas and islet cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of normal mouse intestine frozen section showing membrane and cytoplasmic staining.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of N-Ras expression in HeLa (A) and KNRK (B) whole cell lysates.| Application Notes | recommended for detection of N-Ras p21 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
