Application
Western blot analysis of Hic-5 expression in non-transfected: (A) and mouse Hic-5 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Hic-5 expression in non-transfected: (A) and human Hic-5 transfected: (B) 293T whole cell lysates.| Product Name | Hic-5 Antibody (H-75) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-75 mapping at the N-terminus of Hic-5 of human origin |
| Modification Notes | In addition to paxillin, zysin, LPP, Ajuba and TRIP6, hydrogen-peroxide inducible clone 5 (Hic-5) is a member of the LIM family. Hic-5 contains four LIM motifs and seven zinc finger domains. In the cell, Hic-5 localizes to the nuclear matrix and focal adhesion complexes where the LIM domains mediate the interactions of Hic-5 with focal adhesions. Known also as transforming factor beta 1 induced transcript 1, Hic-5 shares extensive homology with the structural protein paxillin, which is involved in the regulation of focal adhesion dynamics. Hic-5 inhibits integrin-mediated cell spreading on Fibronectin by out competing paxillin for focal adhesion kinase and thereby preventing downstream signal transduction. Increased expression of Hic-5 leads to cellular senescence in developing fibroblasts. During myogenesis, expression of Hic-5 blocks differentiation and induces apoptosis of developing myoblasts. The gene encoding human Hic-5 maps to chromosome 16. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of Hic-5 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-75 mapping at the N-terminus of Hic-5 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-75 mapping at the N-terminus of Hic-5 of human origin |
| Entrez Gene | 7041 |
|---|
Application
Western blot analysis of Hic-5 expression in non-transfected: (A) and mouse Hic-5 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Hic-5 expression in non-transfected: (A) and human Hic-5 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of Hic-5 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
