Application
Antibodies tested include p-ERK 1/2 (Thr 202): (A, B) and ERK 2 (K-23): (C, D).Application
Antibodies tested include p-ERK 1/2 (Thr 202): (A, B, C) and ERK 2 (K-23): (D, E, F).Application
Western blot analysis of ERK 1/2 phosphorylation in untreated (A), PMA-treated (B) and PMA and lambda protein phosphatase treated (C) Jurkat whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear and cytoplasmic staining.Application
Western blot analysis of phosphorylated ERK 1/2 expression in untreated 293 (A), NACI-treated 293 (B) TNFα-treated MCF7 (C) and TNFα-treated HeLa (D) whole cell lysates.| Product Name | p-ERK 1/2 Antibody (Thr 202) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to phosphorylated Thr 202 of ERK 1/2 of human origin |
| Modification | Phosphoralated |
| Modification Notes | The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases, p44 and p42, designated extracellular-signal related kinase 1 (ERK 1) and ERK 2, respectively (1–3). ERK proteins are regulated by dual phosphorylation at Tyrosine 204 and 187 and Threonine 177 and 160 residues mapping within a characteristic Thr-Glu-Tyr motif (4,5). Phosphorylation at both the Threonine 202 and Tyrosine 204 residues of ERK1 and Threonine 185 and Tyrosine 187 residues of ERK2 is required for full enzymatic activation (6). The structural consequences of dual-phosphorylation in the ERK2 include active site closure, alignment of key catalytic residues that interact with ATP, and remodeling of the activation loop (6). In response to activation, MAP kinases phosphorylate downstream components on serine and threonine (4,5). Upstream MAP kinase regulators include MAP kinase kinase (MEK), MEK kinase and Raf-1 (5). The ERK family has three additional members: ERK 3, ERK 5 and ERK 6 (7,8). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC |
| recommended for detection of Thr 202 phosphorylated ERK 1 of human origin and correspondingly phosphorylated Thr 203 of mouse and rat origin and Thr 184 phosphorylated ERK 2 of human origin and correspondingly phosphorylated Thr 183 of mouse and rat origin by WB, IP, IF and IHC(P): |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Thr 202 of ERK 1/2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Thr 202 of ERK 1/2 of human origin |
| Entrez Gene | 5595 |
|---|
Application
Antibodies tested include p-ERK 1/2 (Thr 202): (A, B) and ERK 2 (K-23): (C, D).
Application
Antibodies tested include p-ERK 1/2 (Thr 202): (A, B, C) and ERK 2 (K-23): (D, E, F).
Application
Western blot analysis of ERK 1/2 phosphorylation in untreated (A), PMA-treated (B) and PMA and lambda protein phosphatase treated (C) Jurkat whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear and cytoplasmic staining.
Application
Western blot analysis of phosphorylated ERK 1/2 expression in untreated 293 (A), NACI-treated 293 (B) TNFα-treated MCF7 (C) and TNFα-treated HeLa (D) whole cell lysates.| Application Notes | recommended for detection of Thr 202 phosphorylated ERK 1 of human origin and correspondingly phosphorylated Thr 203 of mouse and rat origin and Thr 184 phosphorylated ERK 2 of human origin and correspondingly phosphorylated Thr 183 of mouse and rat origin by WB, IP, IF and IHC(P): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
