Application
Western blot analysis of 9G8 expression in non-transfected: (A) and mouse 9G8 transfected: (B) 293T whole cell lysates.| Product Name | 9G8/SRp20 Antibody (H-120) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-120 of 9G8 of human origin |
| Modification Notes | Pre-mRNA splicing enhancer elements are short RNA sequences capable of activating weak splice sites in nearby introns, and they are required for accurate splice site recognition and the control of alternative splicing (1). Splicing enhancer elements contain specific binding sites for serine/arginine (SR)-rich splicing factors, which include SC35, 9G8, SRp20, and SF2/ASF (2). The family of SR factors all contain one or more RNA recognition motifs (RRM) and an arginine/serine (RS)-rich domain, and they are essential for constitutive splicing and also regulate splicing in a concentration-dependent manner by influencing the selection of alternative splice sites (3,4). The majority of SR proteins, including SC35 and SRp40, are confined to the nucleus, while SF2/ASF, SRp20, and 9G8 are continuously shuttled between the nucleus and the cytoplasm and contribute to mRNA transport (5). The activity of SR proteins in regulated splicing is antagonized by members of the hnRNP A/B family of proteins, which induce drastic shifts in the selection of splicing-sites (6). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of 9G8 and SRp20 of mouse, rat and human origin by WB, IP, IF and ELISA partially cross-reactive with other pre-mRNA splicing factors also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-120 of 9G8 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-120 of 9G8 of human origin |
| Entrez Gene | 6432 |
|---|
Application
Western blot analysis of 9G8 expression in non-transfected: (A) and mouse 9G8 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of 9G8 and SRp20 of mouse, rat and human origin by WB, IP, IF and ELISA partially cross-reactive with other pre-mRNA splicing factors also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
