Application
Western blot analysis of AF-6 expression in non-transfected: (A) and mouse AF-6 transfected: (B) 293 whole cell lysates.| Product Name | AF-6 Antibody (H-106) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 5-110 mapping near the N-terminus of AF-6 of human origin |
| Modification Notes | The dynamics of a cell-cell interface such as tight junctions or adherens junctions are important in many developmental, physiological, and pathological processes. AF-6 (MLLT4: myeloid/lymphoid or mixed-lineage leukemia trans-located to 4) is a 1,612 amino acid protein that contains two N-terminal Ras binding domains (RBD) and a GLGF motif, and is implicated in Ras-mediated signaling events occurring at peripheral cell-cell junctions. AF-6 interacts with F-actin and Profilin in cell-cell junctions, and may modulate Actin modeling near adhesion complexes. Furthermore, AF-6 coordinates junction adhesion molecule (JAM) recruitment to intercellular junctions through a PDZ domain. Developing mice deficient in AF-6 activity display a loss of neuroepithelial polarity, suggesting that AF-6 activity is an important regulator of cell-cell junctions that influence apical/basolateral asymmetry. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of AF-6 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 5-110 mapping near the N-terminus of AF-6 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 5-110 mapping near the N-terminus of AF-6 of human origin |
| Entrez Gene | 4301 |
|---|
Application
Western blot analysis of AF-6 expression in non-transfected: (A) and mouse AF-6 transfected: (B) 293 whole cell lysates.| Application Notes | recommended for detection of AF-6 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
