Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cervix tissue showing membrane staining of squamous epithelial cells.Application
Western blot analysis of TGase1 expression in non-transfected: (A) and human TGase1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of TGase1 expression in non-transfected 293T: (A), mouse TGase1 transfected 293T: (B) and Caki-1 (C) whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human oral mucosa tissue showing membrane staining of squamous epithelial cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of TGase1 expression in non-transfected: (A) and human TGase1 transfected: (B) 293T whole cell lysates.Application
Immunofluorescence staining of normal mouse kidney frozen section showing cytoplasmic staining.| Product Name | TGase1 Antibody (H-87) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 731-817 mapping at the C-terminus of TGase1 of human origin |
| Modification Notes | Terminally differentiating mammalian epidermal cells acquire an insoluble, 10 to 20 nm thick protein deposit on the intracellular surface of the plasma membrane known as the cross-linked cell envelope (CE). The CE is a component of the epidermis that is generated through formation of disulfide bonds and g-glutamyl-lysine isodipeptide bonds, which are formed by the action of transglutaminases (TGases). TGases are intercellularly localizing, Ca2+-dependent enzymes that catalyze the formation of isopeptide bonds by transferring an amine on to glutaminyl residues, thereby cross-linking glutamine residues and lysine residues in substrate proteins. TGases influence numerous biological processes, including blood coagulation, epidermal differentiation, seminal fluid coagulation, fertilization, cell differentiation and apoptosis. Human keratinocyte transglutaminase (TGase1) is a membrane associated, 817 amino acid protein. Human tissue transglutaminase (TGase2) is an endothelial cell specific, 687 amino acid protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of TGase1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 731-817 mapping at the C-terminus of TGase1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 731-817 mapping at the C-terminus of TGase1 of human origin |
| Entrez Gene | 7051 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cervix tissue showing membrane staining of squamous epithelial cells.
Application
Western blot analysis of TGase1 expression in non-transfected: (A) and human TGase1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of TGase1 expression in non-transfected 293T: (A), mouse TGase1 transfected 293T: (B) and Caki-1 (C) whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human oral mucosa tissue showing membrane staining of squamous epithelial cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of TGase1 expression in non-transfected: (A) and human TGase1 transfected: (B) 293T whole cell lysates.
Application
Immunofluorescence staining of normal mouse kidney frozen section showing cytoplasmic staining.| Application Notes | recommended for detection of TGase1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
