Application
Western blot analysis of melanotransferrin expression in non-transfected 293T: (A), mouse melanotransferrin transfected 293T: (B) and SK-MEL-28 (C) whole cell lysates.| Product Name | melanotransferrin Antibody (H-70) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 601-670 mapping near the C-terminus of melanotransferrin of human origin |
| Modification Notes | Melanotransferrin is a member of the transferrin family of iron-binding proteins, which also includes serum transferrin, lactoferrin, and ovotransferrin, and it is highly expressed on melanoma cells. Melanotransferrin, also designated p97, shares a high degree of homology with transferrin, but does not play a significant role in the uptake of iron. Melanotransferrin utilizes a member of the low-density lipoprotein receptor family for transendothelial transport, which is not as efficient as the transport of transferrin through the corresponding transferrin receptor. The gene encoding human melanotransferrin maps to chromosome 3q28-29, and is predominantly expressed as either a membrane bound protein or a secreted form of the protein. Melanotransferrin is expressed in brain, where it may be involved in Alzheimer’s disease. Melanotransferrin may also protect against membrane-lipid peroxidation, possess a metalloprotease activity, and possibly participate in intracellular adhesion. Further research will be necessary to fully elucidate the functions of this protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of melanotransferrin of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 601-670 mapping near the C-terminus of melanotransferrin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 601-670 mapping near the C-terminus of melanotransferrin of human origin |
| Entrez Gene | 4241 |
|---|
Application
Western blot analysis of melanotransferrin expression in non-transfected 293T: (A), mouse melanotransferrin transfected 293T: (B) and SK-MEL-28 (C) whole cell lysates.| Application Notes | recommended for detection of melanotransferrin of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
