Application
Immunofluorescence staining of methanol-fixed HeLa cells showing perinuclear and cytoplasmic localization.| Product Name | Gα i/o/t/z/gust Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 55-354 mapping at the C-terminus of Gα i-3 of human origin |
| Modification Notes | Heterotrimeric G proteins function to relay information from cell surface receptors to intracellular effectors (1). Each of a very broad range of receptors specifically detects an extracellular stimulus (a photon, pheromone, odorant, hormone or neurotransmitter) while the effectors (e.g., adenyl cyclase), which act to generate one or more intracellular messengers, are less numerous. In mammals, G protein å, ∫ and © polypeptides are encoded by at least 16, 4 and 7 genes, respectively (2–5). Most interest in G proteins has been focused on their å subunits, since these proteins bind and hydrolyze GTP and most obviously regulate the activity of the best studied effectors. Four distinct classes of Ga subunits have been identified; these include Gs, Gi, Gq and Ga 12/13 (3,4). The Gi class comprises all the known å subunits that are susceptible to pertussis toxin modifications, including Gå i-1, Gå i-2, Gå i-3, Gå o, Gå t1, Gå t2, Gå z and Gå gust (4). Of these, the three Ga i subtypes function to open atrial potassium channels (6). Gustducin has been identified as a taste-cell-specific G protein within the Gi subclass of Ga subunit proteins that is most closely related to the transducins and exclusively expressed in taste buds (3). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Gα i-1,2,3, Gα o, Gα t1,2, Gα z and Gα gust of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 55-354 mapping at the C-terminus of G&alpha i-3 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 55-354 mapping at the C-terminus of Gα i-3 of human origin |
| Entrez Gene | 2770 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing perinuclear and cytoplasmic localization.| Application Notes | recommended for detection of Gα i-1,2,3, Gα o, Gα t1,2, Gα z and Gα gust of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
