Application
Western blot analysis of MT-MMP-4 expression in MCF7 (A) and LNCaP (B) whole cell lysates.| Product Name | MT-MMP-4 Antibody (H-110) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 471-580 mapping near the C-terminus of MT-MMP-4 of human origin |
| Modification Notes | The matrix metalloproteinases (MMPs) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin and proteoglycan (1). MMP catalysis requires both calcium and zinc (2). MT-MMP 4 (also known as MMP-17 or MT4-MMP) is a glycosylphosphatidylinositol (GPI)-anchored proteinase. The zinc-dependent MMP has a unique specificity among synthetic substrates and the capability to degrade gelatin and activate progelatinase A (3,4). MT-MMP 4 is mainly expressed in the brain, leukocytes, colon, ovary and testis. In addition, MMP-4 is expressed in all breast carcinomas (5). The human MT-MMP 5 (also known as MMP-24 or MT5-MMP) gene maps to chromosome 20q11.2, a region frequently amplified in tumors. MMP-5 is predominantly expressed in brain, kidney, pancreas and lung. MT-MMP 5 is also expressed at high levels in brain tumors compared to normal brain tissue (6). MT-MMP 6 (also known as MMP-25, MT6-MMP or Leukolysin) is the second GPI-anchored proteinase in the MMP family (7). A C-terminal-truncated MMP-6 protein is expressed as a strong gelatinolytic species that is derived from a cell-associated proenzyme. MT-MMP 6 is expressed in leukocytes, lung and spleen (8). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of MT-MMP-4 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 471-580 mapping near the C-terminus of MT-MMP-4 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 471-580 mapping near the C-terminus of MT-MMP-4 of human origin |
| Entrez Gene | 4326 |
|---|
Application
Western blot analysis of MT-MMP-4 expression in MCF7 (A) and LNCaP (B) whole cell lysates.| Application Notes | recommended for detection of MT-MMP-4 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
