Application
Western blot analysis of ANT1/2/3/4 expression in non-transfected: (A) and mouse ANT1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of ANT1/2/3/4 expression in mouse heart tissue extract.Application
Western blot analysis of ANT1/2/3/4 expression in rat skeletal muscle tissue extract.| Product Name | ANT1/2/3/4 Antibody (H-188) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 45-233 of ANT1 of human origin |
| Modification Notes | Adenine nucleotide translocator (ANT) and the voltage-dependent anion-selective channel proteins 1 and 2 (VDAC1 and VDAC2) are components of the permeability transition pore complex (PTPC) of the mitochondrial inner and outer membranes, respectively. Formation of PTPCs, the subsequent dissipation of mitochondrial inner membrane potential and release of cytochrome c through the outer mitochondrial membrane are critical events in the early stages of apoptosis. Bax, a proapoptotic protein, has been shown to act upon ANT to induce the dissipation of mitochondrial inner membrane potential. ANT1 has a role in the maintenance of mitochondrial DNA by catalyzing the exchange of ADP and ATP across the mitochondrial inner membrane. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of ANT1, ANT2 and ANT4 of mouse, rat and human origin, and ANT3 of human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 45-233 of ANT1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 45-233 of ANT1 of human origin |
| Entrez Gene | 291 |
|---|
Application
Western blot analysis of ANT1/2/3/4 expression in non-transfected: (A) and mouse ANT1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of ANT1/2/3/4 expression in mouse heart tissue extract.
Application
Western blot analysis of ANT1/2/3/4 expression in rat skeletal muscle tissue extract.| Application Notes | recommended for detection of ANT1, ANT2 and ANT4 of mouse, rat and human origin, and ANT3 of human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
