Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human colon tissue showing cytoplasmic staining of glandular cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of Rab 2A expression in non-transfected (A) and human Rab 2A transfected (B) 293T whole cell lysates.Application
Western blot analysis of Rab 2 expression in non-transfected: (A) and human Rab 2 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Rab 2A expression in non-transfected: (A) and human Rab 2A transfected: (B) 293T whole cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.| Product Name | Rab 2 Antibody (FL-212) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-212 representing full length Rab 2A of human origin |
| Modification Notes | The Ras-related superfamily of guanine nucleotide binding proteins includes the R-Ras, Rap, Ral/Rec and Rho/Rab subfamilies all of which are thought to play an important role in either endocytosis or in biosynthetic protein transport. The process of transporting newly synthesized proteins from the Endoplasmic reticulum (ER) to various stacks of the Golgi complex and to secretory vesicles involves the movement of carrier vesicles and requires Rab protein function. Rab proteins are also an integral part of endocytic pathways. A member of the small Rab family and GTPase superfamily, Rab 2, which is also known as Ras-related protein Rab-2A, RAB2A, Ras-related protein Rab-2B or RAB2B, plays an essential in protein transport from the Endoplasmic reticulum to the Golgi complex. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Rab 2A and 2B partially cross-reactive with other Rab family members of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-212 representing full length Rab 2A of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-212 representing full length Rab 2A of human origin |
| Entrez Gene | 5862 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human colon tissue showing cytoplasmic staining of glandular cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of Rab 2A expression in non-transfected (A) and human Rab 2A transfected (B) 293T whole cell lysates.
Application
Western blot analysis of Rab 2 expression in non-transfected: (A) and human Rab 2 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Rab 2A expression in non-transfected: (A) and human Rab 2A transfected: (B) 293T whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.| Application Notes | recommended for detection of Rab 2A and 2B partially cross-reactive with other Rab family members of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
