Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in tubules.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human lung tumor tissue showing cytoplasmic and extracellular localization.Application
Western blot analysis of SP-A expression in Mv 1 Lu (A) and WI 38 (B) whole cell lysates.| Product Name | SP-A Antibody (H-148) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 101-248 mapping at the C-terminus of pulmonary surfactant protein A (SP-A) of human origin |
| Modification Notes | Pulmonary surfactant is primarily responsible for lowering the surface tension at the air-liquid interface in the alveoli, a process that is essential for normal respiration. Pulmonary surfactant is a mixture of phospholipids and proteins, including four distinct surfactant-associated proteins (SPs), SP-A, SP-B, SP-C, SP-D. SP-B and SP-C are predominantly hydrophobic proteins that associate with lipids to promote the absorption of surfactant phospholipids and to reduce the surface tension in the alveoli. SP-A and SP-D are large multimeric proteins belonging to the family of calcium-dependent lectins, designated collectins, which contribute to the innate immune system. Both SP-A and SP-D have been shown to protect against microbial challenge through binding to the lipid components of the bacterial cell wall and facilitating the rapid removal of microbials. In humans, there are four SFTPA genes localized on chromosome 10. Research indicates that the SFTPA genes are differentially regulated by glucocorticoids, insulin, and cAMP. Expression of two highly similar SP-A proteins, SP-A1 and SP-A2 has been confirmed. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of SP-A1 and SP-A2 of mouse, rat and human origin and SP-A of mouse and rat origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including mink: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 101-248 mapping at the C-terminus of pulmonary surfactant protein A (SP-A) of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 101-248 mapping at the C-terminus of pulmonary surfactant protein A (SP-A) of human origin |
| Entrez Gene | 653509 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in tubules.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human lung tumor tissue showing cytoplasmic and extracellular localization.
Application
Western blot analysis of SP-A expression in Mv 1 Lu (A) and WI 38 (B) whole cell lysates.| Application Notes | recommended for detection of SP-A1 and SP-A2 of mouse, rat and human origin and SP-A of mouse and rat origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including mink: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
