Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in tubules.Application
Western blot analysis of arginase II expression in non-transfected: (A) and mouse arginase II transfected: (B) 293T whole cell lysates.Application
Western blot analysis of arginase II expression in non-transfected 293T: (A), human arginase II transfected 293T: (B) and T84 (C) whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human prostate cancer tissue showing cytoplasmic staining of tumor cells (low and high magni?fication). Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of arginase II expression in rat kidney tissue extract.| Product Name | arginase II Antibody (H-64) AC |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 291-354 of arginase II of human origin |
| Modification Notes | Arginase I (also designated liver-type arginase), which is expressed almost exclusively in the liver, catalyzes the conversion of arginine to ornithine and urea (1). The human arginase I gene, which maps to chromosome 6q23, encodes a 322 amino acid protein. Arginase I exists as a homotrimeric protein and contains a binuclear manganese cluster (2-4). Arginase II catalyzes the same reaction as arginase I, but differs in its tissue specificity and subcellular location (5,6). Specifically, arginase II localizes to the mitochondria (5,6). Arginase II is expressed in non-hepatic tissues, with the highest levels of expression in the kidneys, but, unlike arginase I, is not expressed in liver (5,6). The human arginase II gene, which maps to chromosome 14q24.1, encodes a 354 amino acid protein (3,5-7). In addition, arginase II contains a putative amino-terminal mitochondrial localization sequence (5,6). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of arginase II of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 291-354 of arginase II of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 291-354 of arginase II of human origin |
| Entrez Gene | 384 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in tubules.
Application
Western blot analysis of arginase II expression in non-transfected: (A) and mouse arginase II transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of arginase II expression in non-transfected 293T: (A), human arginase II transfected 293T: (B) and T84 (C) whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human prostate cancer tissue showing cytoplasmic staining of tumor cells (low and high magni?fication). Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of arginase II expression in rat kidney tissue extract.| Application Notes | recommended for detection of arginase II of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
