Application
Western blot analysis of Apg-1 expression in HeLa (A) and K-562 (B) whole cell lysates.Application
Western blot analysis of Apg-1 expression in non-transfected 293T: (A), mouse Apg-1 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic staining.Application
Western blot analysis of Apg-1 expression in NIH/3T3 whole cell lysate.| Product Name | Apg-1 Antibody (N-231) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 231-838 of the Apg-1 heat shock protein of mouse origin |
| Modification Notes | The heat shock proteins (HSPs) comprise a group of highly conserved, abundantly expressed proteins with diverse functions, which include the assembly and sequestering of multiprotein complexes, transportation of nascent polypeptide chains across cellular membranes and regulation of protein folding. Heat shock proteins (also known as molecular chaperones) fall into six general families: HSP 90, HSP 70, HSP 60, the low molecular weight HSPs, the immunophilins and the HSP 110 family. The HSP 110 family (also known as the HSP 105 family) is composed of HSP 105, Apg-1 and Apg-2. Apg-1, also known as HSPA4L (heat shock 70 kDa protein 4-like) or Osp94 (osmotic stress protein 94), is an 839 amino acid protein that possesses chaperone activity in vitro, where it inhibits aggregation of citrate synthase. A homodimer, Apg-1 subcellularly localizes to cytoplasm and nucleus, and may translocate to nucleus after heat shock. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Apg-1 and, to a lesser extent, Apg-2 and HSP 105 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 231-838 of the Apg-1 heat shock protein of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 231-838 of the Apg-1 heat shock protein of mouse origin |
| Entrez Gene | 22824 |
|---|
Application
Western blot analysis of Apg-1 expression in HeLa (A) and K-562 (B) whole cell lysates.
Application
Western blot analysis of Apg-1 expression in non-transfected 293T: (A), mouse Apg-1 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic staining.
Application
Western blot analysis of Apg-1 expression in NIH/3T3 whole cell lysate.| Application Notes | recommended for detection of Apg-1 and, to a lesser extent, Apg-2 and HSP 105 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
