Application
Western blot analysis of Ribophorin II expression in NIH/3T3 whole cell lysate.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.| Product Name | Ribophorin II Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 322-631 mapping at the C-terminus of Riborphorin II of human origin |
| Modification Notes | Membrane proteins of the endoplasmic reticulum (ER) may be localized by mechanisms that involve retention, retrieval or a combination of both. ER localization information has been found in cytoplasmic, transmembrane or luminal domains. Specific retrieval mechanisms have been identified for luminal ER proteins, which contain a KDEL domain, and for type I transmembrane proteins carrying a dilysine motif. Mammalian oligosaccharyltransferase (OST) is a protein complex that is composed of four rough ER-specific, type I transmembrane proteins: Ribophorins I and II (RI and RII), OST48 and DAD1 (also designated defender against apoptotic death). The ribophorins are integral membrane glycoproteins that localize exclusively to the rough ER. There is affinity between the cytoplasmically located N-terminal region of DAD1 and the short cytoplasmic tail of OST48 to place DAD1 firmly into the OST complex. The OST complex affects the cotranslational N-glycosylation of newly synthesized polypeptides. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Riborphorin II of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 322-631 mapping at the C-terminus of Riborphorin II of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 322-631 mapping at the C-terminus of Riborphorin II of human origin |
| Entrez Gene | 6185 |
|---|
Application
Western blot analysis of Ribophorin II expression in NIH/3T3 whole cell lysate.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.| Application Notes | recommended for detection of Riborphorin II of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
