Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing cytoplasmic staining of Leydig cells and cells in seminiferous ducts.Application
Western blot analysis of plasminogen expression in human plasma lysate.Application
Western blot analysis of Plasminogen expression in non-transfected: (A) and mouse Plasminogen transfected: (B) 293T whole cell lysates.Application
Western blot analysis of human recombinant plasminogen.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tumor tissue showing extracellular localization.| Product Name | plasminogen Antibody (H-90) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 16-105 mapping near the N-terminus of plasminogen of human origin |
| Modification Notes | Cleavage of the serine proteinase plasminogen to form plasmin is the central event in the dissolution of blood clots by the fibrinolytic system. Within the fibrinolytic cascade, the serine proteinases urokinase-type plasminogen activator (uPA) and tissue-type plasminogen activator (tPA) activate the proenzyme plasminogen by cleaving plasminogen to form the fibrinolytically active enzyme plasmin. The enzyme plasmin consists of a heavy chain of 561 amino acids, which originates from the N-terminus of plasminogen, and a light chain of 230 amino acid residues, which is derived from the C-terminus of plasminogen. Plasmin is a proangiogenic proteinase that is capable of degrading a variety of extracellular matrix proteins and that facilitates endothelial cell migration and angiogenesis. In the presence of free sulfhydryl donors (FSD), plasmin undergoes auto-proteolysis and is converted to the enzyme angiostatin, which blocks angiogenesis and neovascularization and can inhibit the growth of primary and metastatic tumors. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of plasminogen of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 16-105 mapping near the N-terminus of plasminogen of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 16-105 mapping near the N-terminus of plasminogen of human origin |
| Entrez Gene | 5340 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing cytoplasmic staining of Leydig cells and cells in seminiferous ducts.
Application
Western blot analysis of plasminogen expression in human plasma lysate.
Application
Western blot analysis of Plasminogen expression in non-transfected: (A) and mouse Plasminogen transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of human recombinant plasminogen.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tumor tissue showing extracellular localization.| Application Notes | recommended for detection of plasminogen of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
