Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing cytoplasmic localization.Application
Western blot analysis of SOAT1 expression in non-transfected 293T: (A), human SOAT1 transfected 293T: (B) and THP-1 (C) whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human adrenal gland tissue showing cytoplasmic staining of cortical cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of SOAT1 expression in non-transfected control (A) and SOAT1 transfected (B) WEHI-231 cells. Blot probed with SOAT1 (H-125): . β Tubulin (D-10): used as specificity and loading control.Application
Immunofluorescence staining of methanol-fixed KNRK cells showing cytoplasmic localization.Application
Western blot analysis of SOAT1 expression in rat kidney extract.| Product Name | SOAT1 Antibody (H-125) AC |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-125 mapping at the N-terminus of SOAT1 of human origin |
| Modification Notes | SOAT1 (sterol O-acyltransferase-1), also designated ACAT1, is a homotetra-meric enzyme that catalyzes the formation of cholesterol esters from cholesterol and long chain fatty acyl-coenzyme A (acyl-CoA). The gene encoding human SOAT1 maps to chromosome 1 and is expressed as a protein that localizes to the endoplasmic reticulum (ER) in several tissues, including liver, kidney, adrenal glands and macrophages. SOAT1 is involved in cellular cholesterol homeostasis as well as in foam cell formation and the subsequent progression of atherosclerosis. Several SOAT inhibitors have been developed for the treatment of atherosclerosis. SOAT2 (sterol O-acyltransferase-2), also known as ACAT2 (acyl-CoA:cholesterol acyltransferase-2), participates in lipo-protein assembly, catalyzing cholesterol esterification in mammalian cells. SOAT2 is an integral membrane protein that localizes to the endoplasmic reticulum of human intestinal cells. SOAT2 deficiency contributes to severe mental retardation and hypotonus. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of SOAT1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-125 mapping at the N-terminus of SOAT1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-125 mapping at the N-terminus of SOAT1 of human origin |
| Entrez Gene | 6646 |
|---|
Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing cytoplasmic localization.
Application
Western blot analysis of SOAT1 expression in non-transfected 293T: (A), human SOAT1 transfected 293T: (B) and THP-1 (C) whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human adrenal gland tissue showing cytoplasmic staining of cortical cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of SOAT1 expression in non-transfected control (A) and SOAT1 transfected (B) WEHI-231 cells. Blot probed with SOAT1 (H-125): . β Tubulin (D-10): used as specificity and loading control.
Application
Immunofluorescence staining of methanol-fixed KNRK cells showing cytoplasmic localization.
Application
Western blot analysis of SOAT1 expression in rat kidney extract.| Application Notes | recommended for detection of SOAT1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
