Application
Western blot analysis of Lsh expression in non-transfected 293T: (A), human Lsh transfected 293T: (B) and JM1 (C) whole cell lysates.Application
Western blot analysis of Lsh expression in non-transfected 293T: (A), mouse Lsh transfected 293T: (B) and JM1 (C) whole cell lysates.Application
Western blot analysis of Lsh expression in CCRF-CEM whole cell lysate.Application
Western blot analysis of Lsh expression in JM1 whole cell lysate.| Product Name | Lsh Antibody (H-240) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-240 mapping at the N-terminus of Lsh of human origin |
| Modification Notes | The SNF2 family of helicases are thought to act as transcriptional regulators by their ability to alter the structure of chromatin. One such member, Lymphoid-Specific Helicase (Lsh, also designated Hells for lymphoid-specific DNA helicase), is highly expressed in lymphoid precursor cells in adult animals and is required for the proliferation of peripheral T lymphocytes. Lsh is also expressed in fetal liver and more abundantly in fetal thymus. Lsh protein shows substantial homology to other members of the SNF2 family that are involved in chromatin remodeling and transcription, however does not show similarity to members involved in DNA repair or recombination. The similarity of Lsh to another SNF2 homolog, Mi-2, which functions as a transcriptional silencer in chromatin remodeling, suggests that Lsh may participate in chromatin repression to regulate transcription, rather than chromatin “opening”. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Lsh of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-240 mapping at the N-terminus of Lsh of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-240 mapping at the N-terminus of Lsh of human origin |
| Entrez Gene | 3070 |
|---|
Application
Western blot analysis of Lsh expression in non-transfected 293T: (A), human Lsh transfected 293T: (B) and JM1 (C) whole cell lysates.
Application
Western blot analysis of Lsh expression in non-transfected 293T: (A), mouse Lsh transfected 293T: (B) and JM1 (C) whole cell lysates.
Application
Western blot analysis of Lsh expression in CCRF-CEM whole cell lysate.
Application
Western blot analysis of Lsh expression in JM1 whole cell lysate.| Application Notes | recommended for detection of Lsh of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
