Application
Antibodies tested include p-ERK 1/2 (Thr 204): (A, B) and ERK 2 (K-23): (C, D).Application
Antibodies tested include p-ERK 1/2 (Tyr 204): (A, B, C) and ERK 2 (K-23): (D, E, F).Application
Western blot analysis of ERK 1/2 phosphorylation in untreated (A) and lambda protein phosphatase treated (B) Hela whole cell lysates.Application
Blots were probed with p-ERK 1/2 (Tyr 204): preincubated with its cognate phosphorylated peptide (A) and p-ERK 1/2 (Tyr 204): (B).Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear and cytoplasmic staining.| Product Name | p-ERK 1/2 Antibody (Tyr 204) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to phosphorylated Tyr 204 of ERK 1/2 of human origin |
| Conjugation | Agarose |
| Modification | Phosphoralated |
| Modification Notes | The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases, p44 and p42, designated extracellular-signal related kinase 1 (ERK 1) and ERK 2, respectively (1–3). ERK proteins are regulated by dual phosphorylation at Tyrosine 204 and 187 and Threonine 177 and 160 residues mapping within a characteristic Thr-Glu-Tyr motif (4,5). Phosphorylation at both the Threonine 202 and Tyrosine 204 residues of ERK1 and Threonine 185 and Tyrosine 187 residues of ERK2 is required for full enzymatic activation (6). The structural consequences of dual-phosphorylation in the ERK2 include active site closure, alignment of key catalytic residues that interact with ATP, and remodeling of the activation loop (6). In response to activation, MAP kinases phosphorylate downstream components on serine and threonine (4,5). Upstream MAP kinase regulators include MAP kinase kinase (MEK), MEK kinase and Raf-1 (5). The ERK family has three additional members: ERK 3, ERK 5 and ERK 6 (7,8). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IFIHCIPWB |
| WB:1:100~1:500 IP:1:20~1:100 IF:1:20~1:100 IHC:1:10~1:50 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Tyr 204 of ERK 1/2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Tyr 204 of ERK 1/2 of human origin |
| Entrez Gene | 5595 |
|---|
Application
Antibodies tested include p-ERK 1/2 (Thr 204): (A, B) and ERK 2 (K-23): (C, D).
Application
Antibodies tested include p-ERK 1/2 (Tyr 204): (A, B, C) and ERK 2 (K-23): (D, E, F).
Application
Western blot analysis of ERK 1/2 phosphorylation in untreated (A) and lambda protein phosphatase treated (B) Hela whole cell lysates.
Application
Blots were probed with p-ERK 1/2 (Tyr 204): preincubated with its cognate phosphorylated peptide (A) and p-ERK 1/2 (Tyr 204): (B).
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear and cytoplasmic staining.| Application Notes | WB:1:100~1:500 IP:1:20~1:100 IF:1:20~1:100 IHC:1:10~1:50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
