Application
Western blot analysis of eIF2α expression in non-transfected 293T: (A), mouse eIF2α transfected 293T: (B), K-562 (C) and HeLa (D) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of eIF2α expression in HeLa (A), PMA-treated K-562 (B), KNRK (C) and NIH/3T3 (D) whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast tissue showing nuclear and cytoplasmic staining.Application
Western blot analysis of eIF2α expression in HeLa (A), K-562 (B), KNRK (C) and NIH/3T3 (D) whole cell lysates.| Product Name | eIF2α Antibody (FL-315) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-315 of eIF2α of human origin |
| Modification Notes | The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. The eukaryotic initiation complex is composed of three subunits, designated eIF2å, eIF2ß and eIF2© (eukaryotic translation initiation factor 2 å, ß and ©, respectively), all of which work in concert to form a ternary complex with GTP and tRNA in the early stages of protein synthesis. eIF2å, also known as EIF2S1 or EIF2, is a 315 amino acid subunit of the eukaryotic initiation complex that functions to bind tRNA to the 40S ribosomal subunit (in a GTP-dependent manner), thereby initiating translation. In addition, the phosphorylation state of eIF2å controls the rate of tRNA translation. When eIF2å is not phosphorylated, translation occurs at a normal rate. However, upon phosphorylation by one of several kinases, eIF2å is stabilized, thus preventing the GDP/GTP exchange reaction and slowing translation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of eIF2α of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-315 of eIF2&alpha of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-315 of eIF2α of human origin |
| Entrez Gene | 1965 |
|---|
Application
Western blot analysis of eIF2α expression in non-transfected 293T: (A), mouse eIF2α transfected 293T: (B), K-562 (C) and HeLa (D) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of eIF2α expression in HeLa (A), PMA-treated K-562 (B), KNRK (C) and NIH/3T3 (D) whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast tissue showing nuclear and cytoplasmic staining.
Application
Western blot analysis of eIF2α expression in HeLa (A), K-562 (B), KNRK (C) and NIH/3T3 (D) whole cell lysates.| Application Notes | recommended for detection of eIF2α of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
