Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.Application
Western blot analysis of Gα i-3 expression in mouse brain tissue extract.Application
Western blot analysis of Gα i-3 expression in non-transfected 293T: (A), human Gα i-3 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of Gα i expression in cow brain (A) and rat brain (B) extracts.| Product Name | Gα i-3 Antibody (C-10) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of Gα i-3 of rat origin |
| Modification Notes | Heterotrimeric G proteins function to relay information from cell surface receptors to intracellular effectors (1). Each of a very broad range of receptors specifically detects an extracellular stimulus (a photon, pheromone, odorant, hormone or neurotransmitter) while the effectors (i.e., adenylyl cyclase), which act to generate one or more intracellular messengers, are less numerous. In mammals, G protein å, ∫ and © polypeptides are encoded by at least 16, 4 and 7 genes, respectively (2-5). Most interest in G proteins has been focused on their a subunits, since these proteins bind and hydrolyze GTP and most obviously regulate the activity of the best studied effectors. Four distinct classes of Gå subunits have been identified; these include Gs, Gi, Gq and Gå 12/13 (3,4). The Gi class comprises all the known a subunits that are susceptible to pertussis toxin modifications, including Gå i-1, Gå i-2, Gå i-3, Gå o, Gå t1, Gå t2, Gå z and Gå gust(4). Of these, the three Ga i subtypes function to open atrial potassium channels (6). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Gα i-1 and Gα i-3, and, to a lesser extent, Gα i-2 of mouse, rat, human and bovine origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of G&alpha i-3 of rat origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of Gα i-3 of rat origin |
| Entrez Gene | 2773 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.
Application
Western blot analysis of Gα i-3 expression in mouse brain tissue extract.
Application
Western blot analysis of Gα i-3 expression in non-transfected 293T: (A), human Gα i-3 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of Gα i expression in cow brain (A) and rat brain (B) extracts.| Application Notes | recommended for detection of Gα i-1 and Gα i-3, and, to a lesser extent, Gα i-2 of mouse, rat, human and bovine origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
