Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human skin tissue showing membrane staining of epidermal cells.Application
Western blot analysis of HCAM expression in HUV-EC-C (A), human PBL (B), HeLa (C), U-937 (D) and WI 38 (E) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane staining.Application
Western blot analysis of HCAM expression in HeLa (A), HUV-EC-C (B) and U-937 (C) whole cell lysates.| Product Name | HCAM Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 21-320 mapping within the extracellular domain of HCAM of human origin |
| Modification Notes | Cell adhesion molecules (CAMs) are a family of closely related, cell surface glycoproteins that are involved in cell-cell interactions and are thought to play an important role in embryogenesis and development. HCAM, also known as CD44, LHR, MDU2, MDU3, MIC4, Pgp1, HCELL, MUTCH-I or ECMR-III, is a 742 amino acid single-pass type I membrane protein that is involved in hematopoiesis, lymphocyte activation and tumor metastasis. Functioning as a receptor for hyaluronic acid (HA) and interacting with ligands such as osteopontin (OPN), HCAM mediates both cell-cell and cell-matrix interactions, thereby playing an essential role in cell adhesion and cell migration. HCAM contains one Link domain and, due to alternative splicing events, is expressed as multiple isoforms, some of which are designated CD44R, CDw44, CD44S, CD44H (hematopoietic) and CD44E (epithelial). While most of the HCAM splice varients are expressed in tissues throughout the body, one specific isoform, namely CD44H, is expressed at high levels in cancer tissue, suggesting an important role for the CD44H splice varient in tumor progression. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of HCAM (also designated CD44) of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 21-320 mapping within the extracellular domain of HCAM of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 21-320 mapping within the extracellular domain of HCAM of human origin |
| Entrez Gene | 960 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human skin tissue showing membrane staining of epidermal cells.
Application
Western blot analysis of HCAM expression in HUV-EC-C (A), human PBL (B), HeLa (C), U-937 (D) and WI 38 (E) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane staining.
Application
Western blot analysis of HCAM expression in HeLa (A), HUV-EC-C (B) and U-937 (C) whole cell lysates.| Application Notes | recommended for detection of HCAM (also designated CD44) of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
