Application
Western blot analysis of BHMT expression in mouse kidney tissue extract.| Product Name | BHMT Antibody (H-46) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 361-406 mapping at the C-terminus of BHMT of human origin |
| Modification Notes | Betaine-homocysteine methyltransferase (BHMT) is a zinc-dependent cytosolic protein that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. BHMT is highly expressed in rat liver, and its expression is regulated by dietary methionine and choline. In humans, S-adenosylmethionine (SAM) down-regulates BHMT expression by inducing NF˚B, which acts as a repressor for the BHMT gene. Lowered BHMT levels can lead to ER (endoplasmic reticulum) stress. Mutations in the gene encoding for BHMT may lead to hyperhomocysteinemia, a medical condition characterized by abnormally large amounts of homocysteine in the blood which may be a risk factor for cardiovascular and cerebrovascular diseases. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of BHMT of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 361-406 mapping at the C-terminus of BHMT of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 361-406 mapping at the C-terminus of BHMT of human origin |
Application
Western blot analysis of BHMT expression in mouse kidney tissue extract.| Application Notes | recommended for detection of BHMT of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
