Application
Western blot analysis of Glycophorin C/D expression in K-562 whole cell lysate.Application
Western blot analysis of Glycophorin C expression in non-transfected: (A) and human Glycophorin C transfected: (B) 293T whole cell lysates.| Product Name | Glycophorin C/D Antibody (H-85) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-85 mapping at the N-terminus of Glycophorin C of human origin |
| Modification Notes | Glycophorins A, B and C are sialoglycoproteins of the human erythrocyte membrane, which bear the antigenic determinants for the MN, Ss and Gerbich blood groups, respectively. Glycophorins span the membrane once and present their amino-terminal end to the extracellular surface of the human erythrocyte. The genetic array of expressed glycophorin surface antigens on erythrocytes defines the blood group phenotype of the individual. The human Glycophorin A gene maps to chromosome 4q31.21, contains 7 exons which are 97% homologous to Glycophorin B and encodes a 150 amino acid protein. The human Glycophorin B gene also maps to chromosome 4q31.21 and encodes a 91 amino acid protein. The human Glycophorin C gene maps to chromosome 2q14.3 and contains four exons. Glycophorin C transcript can generate two protein isoforms. Isoform 1 includes all 4 exons and encodes the full length 128 amino acid Glycophorin C protein. Isoform 2, also known as Glycophorin D, is missing exon 2 and encodes a 109 amino acid protein, which specifies the Yus subtype of the Gerbich phenotype. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Glycophorin C and D of human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-85 mapping at the N-terminus of Glycophorin C of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-85 mapping at the N-terminus of Glycophorin C of human origin |
| Entrez Gene | 2995 |
|---|
Application
Western blot analysis of Glycophorin C/D expression in K-562 whole cell lysate.
Application
Western blot analysis of Glycophorin C expression in non-transfected: (A) and human Glycophorin C transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of Glycophorin C and D of human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
