Application
Western blot analysis of SH2D1A expression in non-transfected 293T: (A), human SH2D1A transfected 293T: (B) and Jurkat (C) whole cell lysates and mouse thymus tissue extract (D).| Product Name | SH2D1A Antibody (FL-128) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-128 representing full length SH2D1A of human origin |
| Modification Notes | SH2D1A, also SH2 domain protein 1A, SAP and CD150/SLAM (signaling lymphocyte activation molecule)-associated protein, influences signaling pathways involving SLAM molecules at the interface between T and B cells. SH2D1A modulates SLAM by blocking the recruitment of tyrosine phosphatase SHP2 to the phosphorylated cytoplasmic domain of SLAM. SLAM activation mediates expansion of activated T cells during immune responses, induces production of interferon-© and changes the functional profile of subsets of T cells. SH2D1A is a hydrophilic, 128 amino acid protein that is 96% homologous to the mouse protein in both SH2 and tail domains. SH2D1A is present in all major subsets of T cells, including CD4+, CD45RO+, CD45RA+ and CD8+, but not in B cells. SH2D1A can interact via an SH2 domain with a motif (TIYXXV) present in the cytoplasmic tail of cell-surface receptors SLAM (CD150), CD84, CD229 (LY9) and CD244 (2B4). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of SH2D1A of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-128 representing full length SH2D1A of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-128 representing full length SH2D1A of human origin |
| Entrez Gene | 4068 |
|---|
Application
Western blot analysis of SH2D1A expression in non-transfected 293T: (A), human SH2D1A transfected 293T: (B) and Jurkat (C) whole cell lysates and mouse thymus tissue extract (D).| Application Notes | recommended for detection of SH2D1A of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
